Journal: PLoS ONE
Article Title: The Homeobox Gene MEIS1 Is Methylated in BRAF p.V600E Mutated Colon Tumors
doi: 10.1371/journal.pone.0079898
Figure Lengend Snippet: ( A ) The MEIS1 promoter is hypermethylated in colorectal tumors with a BRAF p.V600E mutation (black dots) when compared to wild type BRAF (white dots). The Y-axis represents the tumor vs. normal log 2 ratio for the median probe per CpG fragment. The horizontal dotted line at log 2 ratio 0 indicates an equal extent of MEIS1 methylation in tumor and normal samples. ( B ) Overview of the analyzed MEIS1 promoter, CpG islands within the promoter and the locus analyzed by MSP primers. Locations were based on the human genome browser (UCSC assembly March 2006, hg18). ( C ) MEIS1 -MSP data showing hypermethylation in BRAF p.V600E colorectal tumors when compared to BRAF wild types. T: tumor; N: normal tissue; M: methylated MEIS1 promoter (168 bp); Um: Unmethylated MEIS1 promoter (176 bp).
Article Snippet: DNA from the 19 colorectal tumors were hybridized on Agilent 244k human CpG island microarrays (Agilent Technologies, Santa Clara, CA, U.S.A.), as described previously [ ].
Techniques: Mutagenesis, Methylation